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THE EFFECT OF BONE BANK PRESERVATION ON THE CALCIFYING MECHANISM

Leroy S. Lavine 1; Martin Burger 2; ; and Albert E. Sobel 1

1 The Department of Surgery, Division of Orthopaedic Surgery, State University of New York College of Medicine at New York City, the Department of Biochemistry, Jewish Hospital of Brooklyn, New York
2 The Department of Surgery, Division of Orthopaedic Surgery, State University of New York College of Medicine at New York City, and the Department of Biochemistry, Jewish Hospital of Brooklyn, New York

1. The influence of various methods of preserving bone on the calcifying mechanism of the tibial epiphysial cartilage of rachitic rats was studied. An in vitro calcification technique was employed.

2. Preservation by deep freezing, aqueous merthiolate, boiling, or acetone inactivated the calcifying mechanism.

3. The inactivation was reversed with calcium ions, most readily in the deep-frozen bones, less readily in the merthiolate treated bones, still less readily in the acetone stored bones, and least in boiled bones.

4. Exposure to calcium ions before preservation in the deep-freeze chest prevented inactivation.

5. The inactivation, reactivation and survival of the calcifying mechanism were confirmed by metachromasia studies.

6. The theory of the mode of action of calcium chloride on reactivation and survival of the calcifying mechanism is presented.
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Hip, Knee, Trauma, Upper limb, Foot & Ankle, Paediatrics, Oncology, Spine, Arthroplasty, General