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Journal of Bone and Joint Surgery - British Volume, Vol 74-B, Issue 2, 284-286
Copyright © 1992 by British Editorial Society of Bone and Joint Surgery

Articles

Culture of human osteoblasts on demineralised human bone. Possible means of graft enhancement

PC Nolan, RM Nicholas, BJ Mulholland, RA Mollan, and DJ Wilson

School of Biomedical Science, Queen's University of Belfast, Northern Ireland.

We cultured human osteoblasts from trabecular bone explants and confirmed their phenotype by alkaline phosphatase assay, increased cyclic adenosine monophosphate production in response to prostaglandin E2 and radiographic micro-analysis of nodules of calcification. The osteoblasts were seeded on to demineralised human bone fragments and examined at ten-day intervals over a 50-day period by scanning electron microscopy. During this time the bank bone became progressively repopulated by the cultured osteoblasts. This system may offer a means of graft enhancement in elective orthopaedic and maxillofacial surgery by delivery of cultured autologous human osteoblasts to bone defects.




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Hip, Knee, Trauma, Upper limb, Foot & Ankle, Paediatrics, Oncology, Spine, Arthroplasty, General