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Journal of Bone and Joint Surgery - British Volume, Vol 84-B, Issue 6,
920-930.
doi: 10.1302/0301-620X.84B6.12457 Copyright © 2002 by British Editorial Society of Bone and Joint Surgery Effects of alumina and zirconium dioxide particles on arachidonic acid metabolism and proinflammatory interleukin production in osteoarthritis and rheumatoid synovial cellsB. Liagre, PhD, Assistant Professor1; S. Moalic, PhD1; P. Vergne, PhD, MD2; J. L. Charissoux, PhD, MD3; D. Bernache-Assollant, PhD, Professor of Physics4; and J. L. Beneytout, PhD, Professor of Biochemistry1
1 Laboratoire de Biochimie, UPRES EA 1085, Faculté de Pharmacie, 2 rue du Docteur Marchland, 87025 Limoges Cedex, France. Correspondence should be sent to Dr J. L. Charissoux. We describe a model which can be used for in vitro biocompatibility assays of biomaterials. We studied the in vitro response of human osteoarthritis or rheumatoid arthritis fibroblast-like synoviocytes to Al2O3 or ZrO2 particles by analyzing the production of interleukin-1 (IL-1) and interleukin-6 (IL-6) and the metabolism of arachidonic acid via lipoxygenase and cyclo-oxygenase pathways. Our results show that, in these cells and under our experimental conditions, Al2O3 and ZrO2 did not significantly modify the synthesis of IL-1 and IL-6 or the metabolism of arachidonic acid.
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