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Journal of Bone and Joint Surgery - British Volume, Vol 85-B, Issue 7,
1077-1083.
doi: 10.1302/0301-620X.85B7.13672 Copyright © 2003 by British Editorial Society of Bone and Joint Surgery Histological evaluation of chondral defects after autologous chondrocyte implantation of the kneeT. W. R. Briggs, MCh Orth, Consultant Orthopaedic Surgeon1; S. Mahroof, MRCS, Specialist Registrar, Orthopaedics and Trauma Surgery1; L. A. David, MRCS, Specialist Registrar, Orthopaedics and Trauma and Surgery1; J. Flannelly, PhD, Research Associate2; J. Pringle, FRCS, Consultant Histopathologist1; and M. Bayliss, PhD, Professor of Connective Tissue Biochemistry2
1 Bone Tumour Unit, Royal National Orthopaedic Hospital Trust, Brockley Hill, Stanmore, Middlesex HA7 4LP, UK. Correspondence should be sent to Mr T. W. R. Briggs. We have performed a prospective, single-surgeon study analysing the histological results of autologous chondrocyte implantation. Fourteen patients underwent autologous chondrocyte implantation of the knee and were evaluated at one year by clinical assessment and arthroscopy. Standard staining was used to examine the sections. In addition, in situ hybridisation was used to establish type-IIa and type-IIb collagen mRNA expression and immunolocalisation techniques demonstrated the positions of type-II and type-X collagen. Eight patients regenerated hyaline cartilage and also contained type-X collagen in the deepest layers and type-II collagen in the deep layers. Three demonstrated fibrocartilage and had type-II collagen in the deep layers. In situ hybridisation revealed that all 14 samples had the potential to express both type-IIa and type-IIb collagen. We have shown that one year after the initial implantation chondrocytes are capable of producing type-II collagen and that they continue to proliferate and mature. This article has been cited by other articles:
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