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Journal of Bone and Joint Surgery - British Volume, Vol 85-B, Issue 8, 1190-1195.
doi: 10.1302/0301-620X.85B8.13516  
Copyright © 2003 by British Editorial Society of Bone and Joint Surgery
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Expression of CCAAT/enhancer-binding protein-beta (C/EBPß) and CHOP in the murine growth plate

TWO POSSIBLE KEY MODULATORS OF CHONDROCYTE DIFFERENTIATION

A. Martos-Rodríguez, PhD, Research Assistant1; I. Santos-Alvarez, PhD, Research Assistant1; V. Campo-Ruíz, MD, Research Fellow2; S. González, PhD, MD, Assistant Professor2; J. P. García-Ruiz, PhD, Adjunct Professor3; and E. Delgado-Baeza, PhD, MD1

1 Orthopedic Research, Unidad de Histologia, Departamento de Morfología, Facultad de Medicina
2 Wellman Laboratories of Photomedicine, Massachusetts General Hospital, Harvard University, 55 Fruit Street, Boston, Massachusetts 02114, USA.
3 Departamento de Biologia Molecular, Centro de Biologia Molecular Severo Ochoa, Facultad de Ciencias Universidad Autónoma de Madrid, Cantoblanco, E-28049 Madrid, Spain.

Correspondence should be sent to Dr E. Delgado-Baeza.

Our aim was to evaluate the expression of transcription factors CCAAT/enhancer-binding protein-beta (C/EBPß) and C/EBP-homologous protein (CHOP) in the growth plate. Proximal tibial epiphyseal growth plates from ten 15-day-old Wistar rats were used. Additionally, anti-proliferating cell nuclear antigen (PCNA), anti-5-bromo-2’-deoxyuridine (BrdU) immunostaining, terminal transferase dUTP nick end-labelling (TUNEL) and nucleolar organiser region-associated proteins (AgNOR) techniques were peformed. The histological morphology of the growth plate from C/EBPßknock-out mice was also analysed.

The normal growth plate showed that C/EBPß and CHOP factors are expressed both in the germinative/ upper proliferative and in the lower proliferative zones. Furthermore, BdrU+ and PCNA+ cells were present exclusively in the germinative and proliferative zones, while TUNEL+ and AgNOR+ cells were seen in all three zones of the growth plate. Acellular areas, hypocellularity, the increase in cell death and anomalies in the architecture of the cell columns were observed in the growth plates of C/EBPß (–/ –) knockout mice.

We suggest that C/EBPß and CHOP transcription factors may be key modulators participating in the chondrocyte differentiation process in the growth plate.






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Hip, Knee, Trauma, Upper limb, Foot & Ankle, Paediatrics, Oncology, Spine, Arthroplasty, General