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Journal of Bone and Joint Surgery - British Volume, Vol 87-B, Issue 10, 1426-1433.
doi: 10.1302/0301-620X.87B10.16160  
Copyright © 2005 by British Editorial Society of Bone and Joint Surgery
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Motility and growth of human bone-marrow mesenchymal stem cells during ex vivo expansion in autologous serum

T. Kobayashi, MD, Graduate Student1; H. Watanabe, MD, PhD, Associate Professor2; T. Yanagawa, MD, PhD, Medical Staff1; S. Tsutsumi, MD, PhD, Medical Staff1; M. Kayakabe, MD, Graduate Student1; T. Shinozaki, MD, PhD, Assistant Professor1; H. Higuchi, MD, PhD, Assistant Professor1; and K. Takagishi, MD, PhD, Director Professor1

1 Department of Orthopaedic Surgery
2 Department of Physical Therapy, Gunma University Graduate School of Medicine, 3-39-22, Showa, Maebashi, Gunma, 371-8511, Japan.

Correspondence should be sent to Professor H. Watanabe; e-mail: hidewat{at}health.gunma-u.ac.jp

Human bone-marrow mesenchymal stem cells have an important role in the repair of musculoskeletal tissues by migrating from the bone marrow into the injured site and undergoing differentiation. We investigated the use of autologous human serum as a substitute for fetal bovine serum in the ex vivo expansion medium to avoid the transmission of dangerous transfectants during clinical reconstruction procedures.

Autologous human serum was as effective in stimulating growth of bone-marrow stem cells as fetal bovine serum. Furthermore, medium supplemented with autologous human serum was more effective in promoting motility than medium with fetal bovine serum in all cases. Addition of B-fibroblast growth factor to medium with human serum stimulated growth, but not motility. Our results suggest that autologous human serum may provide sufficient ex vivo expansion of human bone-marrow mesenchymal stem cells possessing multidifferentiation potential and may be better than fetal bovine serum in preserving high motility.






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Hip, Knee, Trauma, Upper limb, Foot & Ankle, Paediatrics, Oncology, Spine, Arthroplasty, General