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Journal of Bone and Joint Surgery - British Volume, Vol 87-B, Issue 8,
1157-1163.
doi: 10.1302/0301-620X.87B8.15446 Copyright © 2005 by British Editorial Society of Bone and Joint Surgery The effect of bisphosphonates and titanium particles on osteoblastsAN IN VITRO STUDYB. Peter, PhD, Research Assistant1; P.-Y. Zambelli, MD, Orthopaedic Surgeon2; J. Guicheux, PhD, Senior Scientist, Team Leader3; and D. P. Pioletti, PhD, Senior Scientist, Team Leader1
1 Hôpital Orthopédique de la, Suisse Romande, Avenue Pierre Decker 4, 1005 Lausanne, Switzerland. Correspondence should be sent to Dr D. P. Pioletti; e-mail: dominique.pioletti{at}epfl.ch
In an attempt to increase the life of cementless prostheses, an hydroxyapatite-coated implant which releases a bisphosphonate has been suggested as a drug-delivery system. Our in vitro study was designed to determine the maximum dose to which osteoblasts could be safely exposed. Our findings demonstrated that zoledronate did not impair the proliferation of human osteoblasts when used at concentrations below 1 µM. Murine cells can be exposed to concentrations as high as 10 µM. A concentration of 0.01% of titanium particles did not impair the proliferation of either cell line. Zoledronate affected the alkaline phosphatase activity of murine osteoblasts through a chelation phenomenon. The presence of titanium particles strongly decreased the alkaline phosphatase activity of murine osteoblasts. We did not detect any synergic effect of zoledronate and titanium particles on the behaviour of both human and murine osteoblasts.
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