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Journal of Bone and Joint Surgery - British Volume, Vol 88-B, Issue 1, 129-133.
doi: 10.1302/0301-620X.88B1.16795  
Copyright © 2006 by British Editorial Society of Bone and Joint Surgery
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Osteogenic potential of cells in vitro derived from haemarthrosis of the knee induced by injury to the anterior cruciate ligament

S. Y. Lee, MD, Resident1; M. Miwa, MD, PhD, Assistant Professor1; Y. Sakai, MD, PhD, Orthopaedic Surgeon1; R. Kuroda, MD, PhD, Assistant Professor1; T. Niikura, MD, PhD, Orthopaedic Surgeon1; and M. Kurosaka, MD, PhD, Professor and Chairman1

1 Department of Orthopaedic Surgery Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.

Correspondence should be sent to Dr M. Miwa; e-mail: masahiko{at}med.kobe-u.ac.jp

We have investigated whether cells derived from haemarthrosis caused by injury to the anterior cruciate ligament could differentiate into the osteoblast lineage in vitro. Haemarthroses associated with anterior cruciate ligament injuries were aspirated and cultured. After treatment with ß-glycerophosphate, ascorbic acid and dexamethasone or 1,25 (OH)2D3, a significant increase in the activity of alkaline phosphatase was observed. Matrix mineralisation was demonstrated after 28 days and mRNA levels in osteoblast-related genes were enhanced.

Our results suggest that the haemarthrosis induced by injury to the anterior cruciate ligament contains osteoprogenitor cells and is a potential alternative source for cell-based treatment in such injury.




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K. Oe, M. Miwa, Y. Sakai, S. Y. Lee, R. Kuroda, and M. Kurosaka
An in vitro study demonstrating that haematomas found at the site of human fractures contain progenitor cells with multilineage capacity
J Bone Joint Surg Br, January 1, 2007; 89-B(1): 133 - 138.
[Abstract] [Full Text] [PDF]


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Hip, Knee, Trauma, Upper limb, Foot & Ankle, Paediatrics, Oncology, Spine, Arthroplasty, General