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Journal of Bone and Joint Surgery - British Volume, Vol 88-B, Issue 12, 1660-1665.
doi: 10.1302/0301-620X.88B12.17717  
Copyright © 2006 by British Editorial Society of Bone and Joint Surgery
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Anti-apoptotic Bcl-2 gene transfection of human articular chondrocytes protects against nitric oxide-induced apoptosis

S. Surendran, MD, Knee Fellow1; S. H. Kim, PhD, Assistant Professor2; B. K. Jee, PhD, Research Fellow1; S. H. Ahn, MD, PhD, Associate Professor3; P. Gopinathan, MD, Assistant Professor1; and C. W. Han, MD, PhD, Associate Professor1

1 Department of Orthopaedic Surgery, St Mary’s Hospital, Daejeon College of Medicine, The Catholic University of Korea, Daejeon, 301-723 Korea.
2 Department of Molecular Genetics and Biochemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA.
3 Department of Rehabilitation Medicine, College of Medicine, Yeungnam University, Daegu, 705-717 South Korea.

Correspondence should be sent to Dr C. W. Han; e-mail: hancw0523{at}hotmail.com

We stably transfected early passage chondrocytes with an anti-apoptotic Bcl-2 gene in vitro using a retrovirus vector. Samples of articular cartilage were obtained from 11 patients with a mean age of 69 years (61 to 75) who were undergoing total knee replacement for osteoarthritis. The Bcl-2-gene-transfected chondrocytes were compared with non-transfected and lac-Z-gene-transfected chondrocytes, both of which were used as controls. All three groups of cultured chondrocytes were incubated with nitric oxide (NO) for ten days. Using the Trypan Blue exclusion assay, an enzyme-linked immunosorbent assay and flow cytometric analysis, we found that the number of apoptotic chondrocytes was significantly higher in the non-transfected and lac-Z-transfected groups than in the Bcl-2-transfected group (p < 0.05). The Bcl-2-transfected chondrocytes were protected from NO-induced impairment of proteoglycan synthesis.

We conclude that NO-induced chondrocyte death involves a mechanism which appears to be subject to regulation by an anti-apoptotic Bcl-2 gene. Therefore, Bcl-2 gene therapy may prove to be of therapeutic value in protecting human articular chondrocytes.




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J. H. Lee, K. V. B. Prakash, Y. H. Pengatteeri, S. E. Park, H. S. Koh, and C. W. Han
Chondrocyte apoptosis in the regenerated articular cartilage after allogenic chondrocyte transplantation in the rabbit knee
J Bone Joint Surg Br, July 1, 2007; 89-B(7): 977 - 983.
[Abstract] [Full Text] [PDF]


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Hip, Knee, Trauma, Upper limb, Foot & Ankle, Paediatrics, Oncology, Spine, Arthroplasty, General