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Journal of Bone and Joint Surgery - British Volume, Vol 88-B, Issue 4, 544-553.
doi: 10.1302/0301-620X.88B4.16498  
Copyright © 2006 by British Editorial Society of Bone and Joint Surgery
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The culture of articular chondrocytes in hydrogel constructs within a bioreactor enhances cell proliferation and matrix synthesis

M. Akmal, MBBS, BSc, FRCS(Orth and Tr), Consultant Orthopaedic Surgeon1; A. Anand, MBBS, BSc, Foundation year 1 Trainee2; B. Anand, MBBS, BSc, Senior House Officer, Orthopaedics3; M. Wiseman, PhD, Research Fellow4; A. E. Goodship, PhD, MRCVS, Professor of Musculoskeletal Science4; and G. Bentley, MBBS, MCh, FRCS, Emeritus Professor of Orthopaedics4

1 Department of Orthopaedics and Trauma, St. Mary’s Hospital, London W2 1NY, UK.
2 Barnet Hospital, Barnet, Hertfordshire EN5 3DL, UK.
3 Charing Cross Hospital, London W6 8RF, UK.
4 Institute of Orthopaedics, Royal National Orthopaedic Hospital, Brockley Hill, Stanmore, Middlesex HA7 4LP, UK.

Correspondence should be sent to Dr M. Akmal; e-mail: mo.akmal{at}st-marys.nhs.uk

Bovine and human articular chondrocytes were seeded in 2% alginate constructs and cultured for up to 19 days in a rotating-wall-vessel (RWV) and under static conditions. Culture within the RWV enhanced DNA levels for bovine chondrocyte-seeded constructs when compared with static conditions but did not produce enhancement for human cells. There was a significant enhancement of glycosaminoglycans and hydroxyproline synthesis for both bovine and human chondrocytes. In all cases, histological analysis revealed enhanced Safranin-O staining in the peripheral regions of the constructs compared with the central region. There was an overall increase in staining intensity after culture within the RWV compared with static conditions. Type-II collagen was produced by both bovine and human chondrocytes in the peripheral and central regions of the constructs and the staining intensity was enhanced by culture within the RWV. A capsule of flattened cells containing type-I collagen developed around the constructs maintained under static conditions when seeded with either bovine or human chondrocytes, but not when cultured within the RWV bioreactor.






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Hip, Knee, Trauma, Upper limb, Foot & Ankle, Paediatrics, Oncology, Spine, Arthroplasty, General