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Journal of Bone and Joint Surgery - British Volume, Vol 89-B, Issue 1, 133-138.
doi: 10.1302/0301-620X.89B1.18286  
Copyright © 2007 by British Editorial Society of Bone and Joint Surgery
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An in vitro study demonstrating that haematomas found at the site of human fractures contain progenitor cells with multilineage capacity

K. Oe, MD, Orthopaedic Surgeon1; M. Miwa, MD, PhD, Assistant Professor, Orthopaedic Surgeon1; Y. Sakai, MD, PhD, Orthopaedic Surgeon1; S. Y. Lee, MD, Orthopaedic Surgeon1; R. Kuroda, MD, PhD, Lecturer, Orthopaedic Surgeon1; and M. Kurosaka, MD, PhD, Professor and Chairman, Orthopaedic Surgeon1

1 Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, Hyogo 650-0017, Japan.

Correspondence should be sent to Dr M. Miwa; e-mail: masahiko{at}med.kobe-u.ac.jp

We isolated multilineage mesenchymal progenitor cells from haematomas collected from fracture sites. After the haematoma was manually removed from the fracture site it was cut into strips and cultured. Homogenous fibroblastic adherent cells were obtained. Flow cytometry revealed that the adherent cells were consistently positive for mesenchymal stem-cell-related markers CD29, CD44, CD105 and CD166, and were negative for the haemopoietic markers CD14, CD34, CD45 and CD133 similar to bone-marrow-derived mesenchymal stem cells. In the presence of lineage-specific induction factors the adherent cells could differentiate in vitro into osteogenic, chondrogenic and adipogenic cells.

Our results indicate that haematomas found at a fracture site contain multilineage mesenchymal progenitor cells and play an important role in bone healing. Our findings imply that to enhance healing the haematoma should not be removed from the fracture site during osteosynthesis.




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T. Hasegawa, M. Miwa, Y. Sakai, T. Niikura, M. Kurosaka, and T. Komori
Osteogenic activity of human fracture haematoma-derived progenitor cells is stimulated by low-intensity pulsed ultrasound in vitro
J Bone Joint Surg Br, February 1, 2009; 91-B(2): 264 - 270.
[Abstract] [Full Text] [PDF]


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Hip, Knee, Trauma, Upper limb, Foot & Ankle, Paediatrics, Oncology, Spine, Arthroplasty, General