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Journal of Bone and Joint Surgery - British Volume, Vol 89-B, Issue 3, 417-420.
doi: 10.1302/0301-620X.89B3.18491  
Copyright © 2007 by British Editorial Society of Bone and Joint Surgery
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Antibacterial effect of autologous platelet gel enriched with growth factors and other active substances

AN IN VITRO STUDY

T. M. Bielecki, MD, PhD, Orthopaedic Surgeon1; T. S. Gazdzik, MD, PhD, Professor1; J. Arendt, MD, PhD, Professor2; T. Szczepanski, MD, PhD, Pediatrician3; W. Król, MD, PhD, Professor4; and T. Wielkoszynski, MD, PhD, Senior Researcher5

1 Department and Clinic of Orthopaedics, Medical University of Silesia, Pl. Medyków 1, 41-200, Sosnowiec, Poland.
2 Department and Clinic of General and Gastroenterological Surgery, Medical University of Silesia, Ul. Zeromskiego 13, 41-300, Bytom, Poland.
3 Department of Pediatric Hematology and Oncology, Medical University of Silesia, Ul. 3 Maja 13-15, 41-800, Katowice, Poland.
4 Department of Microbiology and Immunology
5 Department of Biochemistry, Medical University of Silesia, Ul. Jordana 19, 41-800, Katowice, Poland.

Correspondence should be sent to Dr T. M. Bielecki; e-mail: tomekbiel{at}o2.pl

Platelet-rich plasma is a new inductive therapy which is being increasingly used for the treatment of the complications of bone healing, such as infection and nonunion. The activator for platelet-rich plasma is a mixture of thrombin and calcium chloride which produces a platelet-rich gel.

We analysed the antibacterial effect of platelet-rich gel in vitro by using the platelet-rich plasma samples of 20 volunteers. In vitro laboratory susceptibility to platelet-rich gel was determined by the Kirby-Bauer disc-diffusion method. Baseline antimicrobial activity was assessed by measuring the zones of inhibition on agar plates coated with selected bacterial strains.

Zones of inhibition produced by platelet-rich gel ranged between 6 mm and 24 mm (mean 9.83 mm) in diameter. Platelet-rich gel inhibited the growth of Staphylococcus aureus and was also active against Escherichia coli. There was no activity against Klebsiella pneumoniae, Enterococcus faecalis, and Pseudomonas aeruginosa. Moreover, platelet-rich gel seemed to induce the in vitro growth of Ps. aeruginosa, suggesting that it may cause an exacerbation of infections with this organism. We believe that a combination of the inductive and antimicrobial properties of platelet-rich gel can improve the treatment of infected delayed healing and nonunion.




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The prophylactic effects of platelet-leucocyte gel in osteomyelitis: AN EXPERIMENTAL STUDY IN A RABBIT MODEL
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[Abstract] [Full Text] [PDF]


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Hip, Knee, Trauma, Upper limb, Foot & Ankle, Paediatrics, Oncology, Spine, Arthroplasty, General