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Journal of Bone and Joint Surgery - British Volume, Vol 89-B, Issue 7, 977-983.
doi: 10.1302/0301-620X.89B7.18983  
Copyright © 2007 by British Editorial Society of Bone and Joint Surgery
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Chondrocyte apoptosis in the regenerated articular cartilage after allogenic chondrocyte transplantation in the rabbit knee

J. H. Lee, MD, Associate Professor1; K. V. B. Prakash, PhD, Fellow2; Y. H. Pengatteeri, MS(Orth), Fellow2; S. E. Park, MD, Instructor2; H. S. Koh, MD, Associate Professor3; and C. W. Han, MD, Associate Professor2

1 Department of Orthopedic Surgery, Chonbuk National University Medical School, San 2-20 Geumam-dong, Deokjin-gu, Jeonju, South Korea 561-180.
2 Department of Orthopedic Surgery, Histotem Seoul Life Foundation Bldg, 518-4, Dunction-dong, Kang Dong-gu, Seoul 134-060, Korea.
3 Department of Orthopedic Surgery, St. Vincent’s Hospital, The Catholic University of Korea, 93-6 Ji-dong, Paldal-gu, Suwon, Gyeonggi-do, Korea 442-723.

Correspondence should be sent to Dr C. W. Han; e-mail: hancw0523{at}hotmail.com

We attempted to repair full-thickness defects in the articular cartilage of the trochlear groove of the femur in 30 rabbit knee joints using allogenic cultured chondrocytes embedded in a collagen gel. The repaired tissues were examined at 2, 4, 8, 12 and 24 weeks after operation using histological and histochemical methods. The articular defect filling index measurement was derived from safranin-O stained sections. Apoptotic cellular fractions were derived from analysis of apoptosis in situ using TUNEL staining, and was confirmed using caspase-3 staining along with quantification of the total cellularity. The mean articular defect filling index decreased with time. After 24 weeks it was 0.7 (SD 0.10), which was significantly lower than the measurements obtained earlier (p < 0.01). The highest mean percentage of apoptotic cells were observed at 12 weeks, although the total cellularity decreased with time. Because apoptotic cell death may play a role in delamination after chondrocyte transplantation, anti-apoptotic gene therapy may protect transplanted chondrocytes from apoptosis.




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Chondrocyte apoptosis in the regenerated articular cartilage
Benedict A Rogers
J Bone Joint Surg Br Online, 5 Sep 2007 [Full text]

(c) British Editorial Society of Bone and Joint Surgery All Rights Reserved
Registered charity no: 209299     Print ISSN: 0301-620X
Hip, Knee, Trauma, Upper limb, Foot & Ankle, Paediatrics, Oncology, Spine, Arthroplasty, General