Logo of The Journal of Bone & Joint Surgery (Br)
Quick search:        
          Advanced Search
Guest Access | Sign In
Journal of Bone and Joint Surgery - British Volume, Vol 89-B, Issue 9, 1261-1267.
doi: 10.1302/0301-620X.89B9.18053  
Copyright © 2007 by British Editorial Society of Bone and Joint Surgery
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow My Folders
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tohyama, H.
Right arrow Articles by Nishihira, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tohyama, H.
Right arrow Articles by Nishihira, J.

The responses of extrinsic fibroblasts infiltrating the devitalised patellar tendon to IL-1ß are different from those of normal tendon fibroblasts

H. Tohyama, MD, PhD, Associate Professor1; K. Yasuda, MD, PhD, Professor1; H. Uchida, MD, PhD, Assistant Professor2; and J. Nishihira, MD, PhD, Professor3

1 Department of Sports Medicine, Hokkaido University School of Medicine, Kita-15 Nishi-7, Sapporo, 060-8638, Japan.
2 Department of Orthopaedic Surgery, Surgical Science Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan.
3 Department of Medical Information, Hokkaido Information University, Nishi-Nopporo, Ebetsu 069-8585, Japan.

Correspondence should be sent to Dr H. Tohyama; e-mail: tohyama{at}med.hokudai.ac.jp

In order to clarify the role of cytokines in the remodelling of the grafted tendon for ligament reconstruction we compared the responses to interleukin (IL)-1ß, platelet-derived growth factor (PDGF)-BB and transforming growth factor (TGF)-ß1 of extrinsic fibroblasts infiltrating the frozen-thawed patellar tendon in rats with that of the normal tendon fibroblasts, in regard to the gene expression of matrix metalloproteinase (MMP)-13, using Northern blot analysis. We also examined, immunohistologically, the local expression of IL-1ß, PDGF-BB, and TGF-ß1 in fibroblasts infiltrating the frozen-thawed patellar tendon.

Northern blot analysis showed that fibroblasts derived from the patellar tendon six weeks after the freeze-thaw procedure in situ showed less response to IL-1ß than normal tendon fibroblasts with respect to MMP-13 mRNA gene expression. The immunohistological findings revealed that IL-1ß was over-expressed in extrinsic fibroblasts which infiltrated the patellar tendon two and six weeks after the freeze-thaw procedure in situ, but neither PDGF-BB nor TGF-ß1 was over-expressed in these extrinsic fibroblasts. Our findings indicated that IL-1ß had a close relationship to matrix remodelling of the grafted tendon for ligament reconstruction, in addition to the commencement of inflammation during the tissue-healing process.




This article has been cited by other articles:


Home page
 J Bone Joint Surg BrHome page
A. P. Rumian, E. R. C. Draper, A. L. Wallace, and A. E. Goodship
The influence of the mechanical environment on remodelling of the patellar tendon
J Bone Joint Surg Br, April 1, 2009; 91-B(4): 557 - 564.
[Abstract] [Full Text] [PDF]


(c) British Editorial Society of Bone and Joint Surgery All Rights Reserved
Registered charity no: 209299     Print ISSN: 0301-620X
Hip, Knee, Trauma, Upper limb, Foot & Ankle, Paediatrics, Oncology, Spine, Arthroplasty, General