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Journal of Bone and Joint Surgery - British Volume, Vol 90-B, Issue 11, 1528-1532.
doi: 10.1302/0301-620X.90B11.21029  
Copyright © 2008 by British Editorial Society of Bone and Joint Surgery
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The cytotoxic effect of phenol and ethanol on the chondrosarcoma-derived cell line OUMS-27

AN IN VITRO EXPERIMENT

S. H. M. Verdegaal, MD, Orthopaedic Surgeon1; W. E. Corver, PhD, Research Scientist2; P. C. W. Hogendoorn, MD, PhD, Professor of Pathology2; and A. H. M. Taminiau, MD, PhD, Professor of Orthopaedics1

1 Department of Orthopaedics
2 Department of Pathology, Leiden University Medical, Center, P. O. Box 9000, J11-R, 2300 RC, Leiden, The Netherlands.

Correspondence should be sent to Dr S. H. M. Verdegaal; e-mail: shmverdegaal{at}hotmail.com

Surgery is considered to be the most effective treatment for cartilaginous tumours. In recent years, a trend has emerged for patients with low-grade tumours to be treated less invasively using curettage followed by various forms of adjuvant therapy. We investigated the potential for phenol to be used as an adjuvant. Using a human chondrosarcoma-derived cartilage-producing cell line OUMS-27 as an in vitro model we studied the cytotoxic effect of phenol and ethanol. Since ethanol is the standard substance used to rinse phenol out of a bone cavity, we included an assessment of ethanol to see whether this was an important secondary factor with respect to cell death. The latter was assessed by flow cytometry.

A cytotoxic effect was found for concentrations of phenol of 1.5% and of ethanol of 42.5%. These results may provide a clinical rationale for the use of both phenol and ethanol as adjuvant therapy after intralesional curettage in low-grade central chondrosarcoma and justify further investigation.






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Hip, Knee, Trauma, Upper limb, Foot & Ankle, Paediatrics, Oncology, Spine, Arthroplasty, General